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Fig. 3. Redistribution of survivin-GFP during cell division. (A) Time-lapse sequence of an LLC-PK cell expressing survivin-GFP during prophase, nuclear envelope breakdown (NEB) and prometaphase. The amount of survivin-GFP at the inner centromeres increases substantially around NEB (time point 0 minutes) and shows further accumulation during prometaphase. The white circle denotes a centromere that remained in focus throughout the observation period. In contrast to inner centromere labeling, the intensity of chromosome arm labeling diminishes during prometaphase after peaking at NEB. A video corresponding to the still images is available as supplementary information (see Movie 1, http://jcs.biologists.org/supplemental/). (B) Changes in the average integrated fluorescence of chromosome arm ( ${blacksquare}$ , n=5) and inner centromere localized survivin-GFP ( ${triangleup}$ , n=8) from late prophase to onset of anaphase. (C) Co-localization of survivin-GFP with the spindle microtubules in anaphase cells is lost by early telophase when survivin-GFP forms separate filamentous accumulations (arrows) that run between the microtubules of the central spindle. The area inside the white boxes is shown in higher magnification. mts, microtubules; surv, survivin-GFP. The merge shows an overlay of DNA (blue), survivin-GFP (green), and spindle microtubules (red). Bars = 10 µm (panel A) and 3 µm (panel C).

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