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Fig. 4. The turnover rate of survivin peaks at early M phase and is partially dependent on microtubule-kinetochore attachment. (A-G) Target areas (indicated with white circles) in HeLa cells (panel A) or LLC-PK cells (panels B-G) were photo-bleached and followed by fluorescence time-lapse microscopy. Pre-bleach and post-bleach images representing partial and maximal recovery are shown. The insets show higher magnification views of the target area. At the end of each row are corresponding graphs of survivin-GFP recovery (arrows indicate the pre-bleach fluorescence level of the target area). Proportions of fluorescence recovery (recf) and half time of recovery (t1/2) are shown for each graph, as percentages. Supplemental Movies 2-6 corresponding to the still images of panels A, B, C, E and F, respectively, are available online (http://jcs.biologists.org/supplemental/).





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