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Fig. 5. Aurora B regulates survivin turnover at inner centromeres. (Top) FRAP analysis in an LLC-PK cell shows that survivin-GFP turnover at metaphase centromeres occurs at its normally rapid rate in a cell treated with the proteaseome inhibitor MG132. (Bottom) In a cell pretreated for 30 minutes with MG132 then treated for 10 minutes with ZM447439 in the continued presence of MG132, survivin-GFP at the inner centromeres shows slow and limited turnover. Pre-bleach and post-bleach images representing partial and maximal recovery are shown. The white circles denote the target area. The graphs show survivin-GFP recovery (arrows indicates the pre-bleach fluorescence level of the target area). Proportion of fluorescence recovery (recf) and half time of recovery (t1/2) are shown as percentages. Movie 7 corresponding to the still images of panel B is available online (http://jcs.biologists.org/supplemental/).





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