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Fig. 1. Absence of ${alpha}$ 3ß1 from keratinocytes leads to increased apoptosis upon serum deprivation. Wild-type MK^+/+ cells (A,B), ${alpha}$ 3-null MK^-/- cells (C,D), or ${alpha}$ 3-transfected MK^-/- cells (E,F) were cultured on LN-5 ECM in serum-free medium for two days. Apoptotic cells were identified by TUNEL staining (A,C,E); corresponding phase images are shown (B,D,F, respectively). Examples of apoptotic cells are indicated with arrows in C and D. (G) Flow cytometry was performed and the percent of TUNEL-positive cells was determined for MK^-/- cells (MK^-/-), or ${alpha}$ 3-transfected MK^-/- cells (MK ${alpha}$ 3) grown in the presence (white bars) or absence (black bars) of serum.

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