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Fig. 2. Absence of 
3ß1 from keratinocytes leads to increased caspase-3 activation upon serum deprivation. (A) Wild-type MK+/+ cells (+/+ lanes), 3-null MK-/- cells (-/- lanes), or 3-transfected MK-/- cells (3 lanes) were cultured on LN-5 ECM for 24 hours in the presence of serum (+serum) or in the absence of serum (serum-free). Cell lysates were assayed for caspase-3 activation by western blot. (B) Cells were kept in suspension for 24 hours in the absence of serum (serum-free, susp.), then assayed for caspase-3 activation as in A. Migratory positions of pro-caspase-3 and cleaved forms of caspase-3 are indicated. Results are representative of more than four independent experiments.
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