QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 3. ${alpha}$ 6ß4-mediated adhesion is not required for keratinocyte survival on LN-5 ECM in the presence of ${alpha}$ 3ß1. ${alpha}$ 3-null MK^-/- cells (A,B), wild-type MK^+/+ cells (C,D), or ${alpha}$ 3-transfected MK^-/- cells (E,F) were seeded onto LN-5 ECM in serum-free medium in the presence of 5 µg/ml of anti- ${alpha}$ 6 blocking antibody GoH3 (B,D,F) or rat IgG_2a isotype control antibody (A,C,E). Adherent cells were photographed approximately two hours after plating. Note that MK^-/- cells fail to spread due to the lack of ${alpha}$ 3ß1 (A), and GoH3 completely eliminated MK^-/- cell adhesion (B). (G) 24 hours after adhesion, the cells shown in panels A and C-F were lysed and assayed for caspase-3 activation by western blot as described in Fig. 2 (upper panels); cell lines and antibody treatments are indicated above the lanes. Filters were stripped and re-probed for keratin 14 (lower panels, K14). Results are representative of two independent experiments, one of which was performed in duplicate.

Return to article