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Fig. 4. ${alpha}$ 3ß1 mediates adhesion-dependent activation of FAK/CAS signaling. Wild-type MK^+/+ cells (+/+ lanes), ${alpha}$ 3-null MK^-/- cells (-/- lanes), or ${alpha}$ 3-transfected MK^-/- cells ( ${alpha}$ 3 lanes) were kept in suspension (suspended) or adhered to LN-5 ECM under serum-free conditions for the times indicated. (A,B) To assay levels of activated FAK, cell lysates were immunoblotted with an antibody against phospho-FAK (Tyr397) (pFAK, upper panel). Blots were stripped and reprobed for total FAK protein (FAK, lower panel). (C) To assay levels of phosphorylated CAS, cell lysates were immunoprecipitated with anti-p130^CAS monoclonal antibody, followed by immunoblotting with monoclonal antibody 4G10 against phospho-Tyr (upper panel). Blots were stripped and reprobed for total CAS protein (lower panel).

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