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Fig. 7. MEK/ERK signaling is required for keratinocyte survival on LN-5 ECM. (A) Wild-type MK+/+ cells or 
3-null MK-/- cells were cultured on LN-5 ECM in serum-free medium in the presence or absence of the MEK inhibitor U0126 (10 µM), as indicated. Cell lysates were assayed for phosphorylated ERK (pERK, upper panel) or total ERK (ERK, lower panel), as described in Fig. 6. Identical results were obtained in cells cultured for 24 or 48 hours. (B) Wild-type MK+/+ cells or 3-null MK-/- cells were cultured on LN-5 ECM in serum-free medium for 48 hours, in the presence or absence of 10 µM U0126, as indicated. Cell lysates were assayed for caspase-3 activation by western blot, as described in Fig. 2. Results are representative of two independent experiments, one of which was performed in duplicate.
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