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Fig. 9. Canalicular secretion of fluorescein-diacetate and cholylglycyl-fluorescein in long-term small-hepatocyte colonies. Cells were cultured for 7 weeks. They were then incubated with fluorescein-dicetate (A) or or cholylglycyl-fluorescein (B). After rinsing, fluorescence analysis demonstrated canalicular fluorescein (A) and cholylglycyl-fluorescein (B) secretion with some fluorescence remaining in hepatocytes. Scale bar, 50 µm.





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