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Fig. 2. Effect of olomoucine on debridement wound healing in organ culture. A 1.5 mm debridement wound was made in corneas of wild-type mice and enucleated eyes were organ cultured for 12 hours in defined medium in the presence or absence of olomoucine (15 µM). Initial wound areas were measured immediately after wounding (20 eyes). To determine the effect of olomoucine, one eye of each animal was cultured with olomoucine and the other eye was cultured without, as a paired control (ten pairs). After 12 hours, eyes were stained and photographed. Each wound area was measured using ImagePro Plus image analysis software (Media Cybernetics, San Diego, CA). (A) Bar graph showing the average area healed as proportion of the original wound area. Wound healing was significantly enhanced by olomoucine (P<0.007). Error bars represent s.e.m. (B) Corneal debridement wound immediately after wounding. (C) Debrided cornea after 12 hours in organ culture. (D) Debrided cornea after 12 hours organ culture in the presence of 15 µM olomoucine.





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