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Fig. 3. Localization of F-actin in wild-type and transgenic corneas during wound healing in organ culture. Debridement wounds were made in corneas of wild-type and ALDH3-Cdk5 transgenic mice. Eyes were placed in organ culture for 12 hours and then fixed and stained with rhodamine-phalloidin. Corneas were dissected and whole mounted for laser scanning confocal fluorescence microscopy. (A) Rhodamine-phalloidin staining of the wound edge in a wild-type cornea. Open arrowheads indicate rows of cells with diffuse actin staining oriented perpendicular to the wound edge. Elongated cells are indicated by solid arrowheads. Points of intense actin staining are often located at the junctions of three or more cells (arrow). (B) Rhodamine-phalloidin staining of the wound edge in an ALDH3-Cdk5 cornea shows cobblestone organization along the wound edge, with few, if any, elongated cells. Cortical actin staining is distributed uniformly around the cell periphery and very few cells show diffuse staining. Bar, 100 µm.
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