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Fig. 3. Subcellular localization of NRADD. Cells were grown on gelatin-coated coverslips, stained with Hoechst dye 33342 (blue) and processed for immunocytochemistry with anti-NRADD antibody (green) and visualized by confocal microscopy. MEFs are mouse embryonic fibroblasts from day 16.5 embryos. Notice the common perinuclear staining (open arrowhead) in MEFs. The edge of the cells are also stained more prominently, particularly structures resembling lamellipodia (closed arrowhead). In the fibroblast line NIH3T3 and the neuroblastoma line N2a, additional staining of nuclear substructure is observed (arrows). All samples were stained in parallel with antigen-blocked antibody. Notice the complete lack of background in the nuclei.





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