spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Analysis of RyR1 expression by immunoblot and RT-PCR. (A) RyR1 detection in microsomal proteins prepared from adult mouse skeletal muscle (SM) (10 µg), and mouse testis (100 µg) at different ages of postnatal development between 10 and 60 days. (B) Immunoblot of microsomal proteins prepared from adult mouse skeletal muscle (SM), 60-day-old testis (T60), 10-day-old testis (T10), mixed populations of germ cells (gc), round spermatids (spt), pachytene spermatocytes (spc), highly purified spermatogonia (spg), epididymal spermatozoa (spz), Sertoli cells (SC). 10 µg of proteins were loaded for SM, whereas 100 µg for all other samples. (C) RTPCR analysis of RyR1 expression in adult skeletal muscle (SM), 60-day-old testis (T60), 7-day-old testis (T7), mixed germ cells (gc), highly purified spermatogonia (spg) and Sertoli cells (SC). Using isoform-specific primers, a single 435 bp product was identified. Lane marked `-' is' the negative control, where cDNA has been omitted in the PCR reaction.





Right arrow Return to article