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Fig. 6. Treatment with 300 µM ryanodine abolishes caffeine induced Ca2+ release in germ cells. Cells were preloaded with 10 mM CaCl2, washed and either incubated (A) with or (B) without 300 µM ryanodine for 1 hour with before measuring caffeine-dependent Ca2+-release. Ionomycin (IMC) was added to elicit further release of intracellular Ca2+. The traces are typical of three independent measurements.





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