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Fig. 1. Puromycin treatment reduces the thapsigargin-induced calcium release from the ER. (A) Traces represent the [Ca2+]c measured in 2 µM fura-2 AM loaded LNCaP cells under control conditions or after a 1-hour incubation with 200 µM puromycin or 1.8 mM cycloheximide. Application of 1 µM thapsigargin (TG) produced an increase in [Ca2+]c as a result of the inhibition of SERCA pumps. All measurements were made at room temperature in Ca2+-free HBSS. (B) Means of the peak values of thapsigargin responses under control conditions (n=34), with 1.8 mM cycloheximide (n=35) and with 200 µM puromycin (n=78).





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