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Fig. 2. Puromycin reduces the free Ca2+ concentration within the ER lumen. (A,B) Typical [Ca2+]ER traces from mag-fura-2 AM loaded LNCaP cells in response to 1 µM ionomycin (IM) (A) and 200 µM puromycin (B). The inset panels show individual fluorescence intensities at 340 nm (Ca2+-insensitive) and 380 nm (Ca2+-sensitive) excitation wavelengths. Note that application of ionomycin results in a drop in the mag-fura-2 ratio. (C) Cumulative data (mean±s.e.m.) for control and puromycin-treated LNCaP cells on the percentage of calcium release from internal stores.





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