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Fig. 1. Generation of an inducible form of FOXN1. (A) Schematic diagram showing the fusion protein. (B,C) Immunofluorescence of 3T3 cells expressing FOXN1ER treated with (B) ethanol or (C) 4OHT for 24 hours and stained with an anti-oestrogen receptor antibody (green) and anti ß-tubulin (red). Scale bar: 30 µm. (D) 3T3 cells transduced with pBabepuro (empty vector), pBabepuroFOXN1 or pBabepuroFOXN1ER were transiently transfected with luciferase reporters WT-FP-luc (black bars) or Mut-FP-luc (yellow bars) and treated with 4OHT as shown. The luciferase activity of each reporter was measured in triplicate and the fold induction calculated. Data shown are from a typical experiment.





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