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Fig. 6. Contrasting effects of FOXN1 and Akt on terminal differentiation. Epidermis was reconstituted by keratinocytes transduced with GFP (A-C), FOXN1ER (D-F), myrAktER (G-I) or A2myrAktER (J-L). (A,D,G,J) Haematoxylin and Eosin staining of paraffin sections. Basal (b), spinous (s), granular (g) and cornified (c) layers are indicated. Sections were stained by immunofluorescence with antibodies to involucrin (B,E,H,K) or loricrin (C,F,I,L) (green). Propidium iodide was used as a nuclear counterstain (red). Scale bar: 50 µm.





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