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Fig. 1. (A) Restriction map of the plasmid rescued from insertional allele cpc1-2, which contains a complete pUC119 vector (shaded) and another small fragment of pUC119 (hatched) embedded within Chlamydomonas genomic sequences (unshaded). (B) Southern blot of genomic DNA from Chlamydomonas wild-type strain 137c and from two insertional alleles of cpc1, digested with PvuII and probed with PstI-MscI fragment labeled 60A8L in (A). Hybridization of this probe to bands that differ from the wild type in both mutants indicates that 60A8L is close to the insertion site in both alleles.





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