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Fig. 6. Western blotting of flagellar axonemes from wild-type cells and three central pair defective Chlamydomonas strains. (A) 6% SDS polyacrylamide gel of wild-type and pf18, pf16 and cpc1 mutant axonemes stained with Coomassie blue. Dots next to the wild-type sample mark two high molecular weight proteins, CP3 and CP4, missing from central pair assembly-defective strain pf18. The sizes of molecular weight standards (in kDa) are shown along the left edge. (B) Western blot of the gel in (A) probed with anti-Cpc1A. A single band is seen in the wild type and at reduced amounts in pf16 axonemes. The immunoreactive band aligns with the location of CP4 in (A).





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