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Fig. 2. Change in the localization of mRNA export factors. The localization of mRNA export factors was monitored in living yeast cells expressing GFP fusion proteins. Cells in exponential phase were treated with ethanol (10% v/v) for 5 minutes or with heat shock stress (42°C) for 60 minutes. Rat7p-GFP and GFP-Rat8p fluorescence were visualized before ethanol treatment (w/o stress) after ethanol treatment for 30 minutes (10% EtOH) and after heat shock (42°C). Lower panels are phase-contrast images of the same cells pictured above.





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