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Fig. 1. Subcellular localisation of EYFP, EYFP(H148G), EYFP-SKL and EYFP(H148G)-SKL expressed in S. cerevisiae and assessed by confocal laser scanning microscopy. Expression of EYFP-SKL (a) and EYFP (H148G)-SKL (b) in wild-type cells results in a peroxisomal localisation of EYFPs, whereas expression of EYFP (c) and EYFP(H148G) (d) results in a cytosolic localisation. Expression of EYFP-SKL (e) and EYFP (H148G)-SKL (f) in pex5{Delta} cells results in a cytosolic localisation, whereas in pex7{Delta} cells both EYFP-SKL (g) and EYFP(H148G)-SKL (h) are localised to peroxisomes. Bar: 5 µM.





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