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Fig. 11. Alfy-like structures are found inside autophagic structures. In order to understand the morphology of Alfy structures more clearly, we treated cells with 50 µM PSI (5-hour pulse followed by 18-hour chase) and prepared them by conventional plastic embedding for EM. We observed filamentous meshworks resembling Alfy-positive structures (asterisk in all micrographs) close to (A, double arrow) or seemingly engulfed by double-membrane structures (B, double arrow). These membranes probably represent ER membranes, as indicated by their continuity with the nuclear envelope (A, arrowheads). The double membranes could form structures resembling autophagosomes (C, double arrow). A more autolysosome-like vesicle with only one limiting membrane (arrowhead) is seen in (D). Abbreviations: Aut, autophagosome; Nu, nucleus.





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