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Fig. 4. Alfy is recruited to cytosolic structures upon starvation. HeLa cells were starved for 0 minutes (A) or 45 minutes (B-D) and thereafter stained with a rabbit anti-Alfy antibody. Scale bar, 10 µm. (E) HeLa cells were starved for different amounts of time in HBSS and the proportion of cells with Alfy-positive structure was calculated based on confocal immunofluorescence using anti-Alfy antibody. Each point represents the mean of three independent experiments. The error bars show s.e.m. P*<0.03 as determined with the {chi}2 test using the SPSS program version 11 (SPSS, Chicago, IL).





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