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Fig. 2. Generation and verification of Z4 mutations. The genomic region with the two alternatively spliced exons of the gene CG12974 (red bars) and the transcript of Z4 depicting its translated regions (blue bars) and untranslated regions (yellow bars) is shown. Arrows indicate the direction of transcription. The site of the EP-element (green triangle) insertion in line EP(3)0756 is shown, from which deletions were generated by imprecise excision to generate the homozygous lethal lines Z4-1.3 and Z4-7.1. The extent of the deletions is indicated by the broken lines. In line Z4-1.3 the deletion encompasses 800 bp of DNA including the 5'-transcription start of Z4 and the 5'-region of the gene CG12974 encoding two exons of the transcript CG12974-RA. In line Z4-7.1 1800 bp to the left of the EP-element are deleted, which removes part of the transcription units of gene CG12974, but leaves the coding region of Z4 unaffected. The bar below indicates the genomic region that complements the lethal mutation of lines Z4-1.3 and Z4-7.1. Within this bar, the vertical line shows the position of the myc-tag that was fused in frame to the 3'-end of the Z4 coding region. Chromosomes from larvae transgenic for the tagged genomic region were stained with DAPI (A) and a monoclonal anti-myc antibody (B). The composite image (C) reveals the localization of the tagged Z4 protein to the chromosomal interbands.
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