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Fig. 6. Binding of Z4 to Notch sequences in vitro. (A) 5'-region of Notch. The black bar represents the transcribed region of Notch, and the gray bar indicates the region deleted in the faswb-allele. The position of the N1 and N2 fragments that were used for in vitro binding to Z4 are shown below. Both fragments were digested and endlabeled at the HinfI-sites indicated. (B) Electrophoretic mobility shift assay with 0.5 µg of purified Z4 (omitted in lanes 1 and 8), 5 ng of the end-labeled DNA fragments N1 (lanes 1-7) or N2 (lanes 8-14) and a 100-, 200-, 400- or 800-fold excess of poly(dI-dC) competitor DNA (lanes 3-6 and 10-13) as indicated on the top.





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