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Fig. 5. A. thaliana SIN-related genes expression in various organs (A) and during the cell cycle (B). (A) The abundance of the AtSGP1, AtSGP2, AtMAP3K ${epsilon}$ 1, AtMAP3K ${epsilon}$ 2, AtMAP4K ${alpha}$ 1 and AtMAP4K ${alpha}$ 2 transcripts was examined in various organs using real-time quantitative PCR. The transcript levels were measured in RNAs extracted from roots (R), rosette leaves (RL), cauline leaves (CL), inflorescence stems (IS), flower buds (FB), mature flowers (MF), siliques (SI) and seedlings (S). Transcript levels are represented as a ratios (R) of the absolute value of the studied gene to the absolute value of the ACT2/ACT8 genes. (B) Analyses of the relative expression levels of AtSGP1, AtSGP2, AtMAP3K ${epsilon}$ 1, AtMAP3K ${epsilon}$ 2, AtMAP4K ${alpha}$ 1 and AtMAP4K ${alpha}$ 2 during the cell cycle. Cultured A. thaliana cells were arrested at the G₁-S phase transition using aphidicolin. After removal of the drug (i.e. time 0), RNA extracts were prepared at the indicated time points and analysed. Accumulation of the G₂-M phase-specific genes Cyc1B1;1 and AtMAP3K ${epsilon}$ 1, and the S phase-specific gene AtH4 were simultaneously monitored. Transcript levels are represented as ratios (R) of the absolute value of the studied gene to the absolute value of each gene at T0.

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