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Fig. 2. Concentration dependence of TSA induced apoptosis. TSA caused an increase in apoptosis in a concentration-dependent manner after incubation for 24 hours, as revealed by the FACS analysis. A fit of the data to a model in which apoptosis reflects the binding of TSA to a single class of independent binding sites (Eqn 2, {alpha}=1) showed a systematic error (dashed line). By contrast, a good fit of the data was obtained with a cooperativity factor of {alpha}=3.2 (solid line).





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