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Fig. 3. Effects of TSA on H2A-YFP expression. (A) H2A-YFP fluorescence intensity increased upon TSA treatment. Cells were incubated with the indicated TSA concentrations for 24 hours and H2A-YFP fluorescence was measured by FACS analysis. The fluorescence intensity of untreated cells was set to 1 and the relative fluorescence increase was plotted. (B) Western blot analysis of H2AYFP protein expression relative to that of lamin. The H2A-YFP concentration increased with longer incubation times (left) and higher TSA concentrations (right).





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