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Fig. 4. TSA-induced changes of interphase chromatin morphology. HeLa cells stably expressing H2A-YFP were treated with 100 ng/ml TSA for 24 hours. 28 µmx28 µm (400x400 pixel) images are shown. Image intensities were normalized to the same average value. (A) Nuclei of control cells imaged in vivo with H2A-YFP. Dense chromatin areas were seen as brighter spots around the nucleolus and close to the nuclear membrane. (B) Fixed and DAPI-stained images of the same nuclei as in A. (C) TSA-treated cells imaged in vivo with H2AYFP. The chromatin distribution was more homogeneous and most of the dense chromatin disappeared. (D) Fixed and DAPI-stained images of the same nuclei as in C.





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