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Fig. 6. Determination of the cell cycle stage of investigated cells. (A) In vivo CLSM images of HeLa cell nuclei expressing H2A-YFP. (B) Phase contrast image of fixed, DAPI-stained cells were taken with an inverse fluorescence microscope. The white circle shows the cell imaged in A. (C) DAPI image of the same area as in B. (D) Comparative histogram of cells analyzed by FACS (solid line) and on coverslips (dashed line). DAPI intensities of the imaged nuclei were plotted and the cell cycle stage of single cells was determined from their position in the histogram.





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