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Fig. 2. In vitro differentiation of pATSCs (A-D), pBMSCs (E-H) along neuronal lineages. Stromal cell-derived neurospheres (A,E) cultured in media supplemented with B27, bFGF and EGF for 4-5 days. Free-floating cell spheres in the NB medium (B,F small inset panel). pATSC and pBMSC-derived neurosphere induced differentiation by culturing on the PDL-Laminin-coated substrate for 10-15 days in B27 supplemented NB media (B-D, F-H). Neurospheres attached to the bottom of the culture dish and protrude extensive cell processes (B,C,F,G). The processes became longer and formed diverse networks 10 days after plating (D,H). Bars, 30 µm (A,C,E); 60 µm (B,D,F,G,H).





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