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Fig. 3. Evaluation of pATSCs-derived single cell clones pluripotency. Single clones-derived progenies were plated at 50 cells/cm2 and induced differentiation to adipogenic, osteogenic and neurogenic. After differentiation cells were stained with Oil Red O and Alzarin Red S for detection of fat and mineralization. For neural lineage detection, cells were immunostained with GFAP, Tuj and MBP antibodies. Positive colonies were counted.





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