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Fig. 8. Comparison of nestin and MAP-2 expression between neural differentiated pATSCs and pBMSCs. (A) Western blot analysis of whole cell lysate of fully differentiated pATSC and pBMSC-derived neurosphere (NS) maintained under neurobasal medium supplemented with B27, bFGF and EGF. Thirty micrograms of protein extract from each cell culture were separated on a 12% acrylamide gel and transferred to polyvinyldiethylfluoride (PVDF) membrane. The blots were probed with antibodies to nestin and MAP2ab followed by HRP-conjugated secondary antibody and developed using enhanced chemiluminescence. (B) Summary of the expression of the MAP2ab mRNA following NS differentiation, as quantified by real-time RT-PCR. The gene expression levels were normalized with respect to endogenous GAPDH.





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