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Fig. 1. Identification and characterization of multiple mouse prominin-1 splice variants. (A) Sites of alternative splicing in prominin-1. The C-terminal sequences of the testis-derived splice variants s3, s4, s5 and s6 are distinct from that of the previously described kidney-derived prominin-1.s1. Arrowheads indicate exon boundaries in the prominin-1 gene. In addition, the s4 variant lacks five amino acid residues in the first extracellular domain, and the s4 and s5 variants both lack a facultative exon in the second extracellular domain (gray arrows, sequences in parentheses). Forks indicate the potential N-glycosylation sites. (B) Genomic organization of part of the murine prominin-1 gene. Exons 24-26 appear as boxes and introns as solid lines. Exons 25a and 25b are in red and green, respectively. The position of the fifth transmembrane domain (TM5) is indicated in black. Arrows mark the position of the prominin-1-specific primers S-TM5 (left) and AS-exon 26 (right) used for PCR analysis. (C) Distinct prominin-1 mRNAs are generated by the use of alternative acceptor sites (s3-s5) and by intron retention (s6). PCR reactions were performed using first-strand cDNA prepared from mouse kidney or testis as template, and the primers indicated in (B). Arrows indicate the three major PCR products and the corresponding prominin-1 mRNA species. , the 3' end of the open reading frame.

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