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Fig. 1. Comparison of Xenopus and human p120 sequences and RT-PCR analysis of p120 expression during early embryonic development and in adult Xenopus tissues. (A) Linear representation of the amino acid sequence similarities between hp120 1ABC and Xp120 isoform 1. In the Xenopus p120 sequence, two translational start sites (M1 at position 1 and M2 at position 75) are indicated. Isoform 2 is derived by splicing, which takes place upstream of M1. A conserved coiled-coil domain in the N-terminal region of p120 is shown (black box). Percentages indicate the similarities of the sequences of Xp120 isoform 1 and hp120 1ABC for the whole protein (calculated with Needleman-Wunsch algorithm from EMBOSS) and for the N-terminal domain (NTD), the central domain (ARM) or the C-terminal domain (CTD) (calculated with the Clustal W method from the GCG program, BEN, Brussels). The Xenopus p120 sequence data are available from EMBL/GenBank under accession numbers AF150743 and AF150744. (B) A semi-quantitative RT-PCR evaluation of Xp120 isoform expression in embryos at different developmental stages and (C) in selected tissues derived from an adult frog. The PCR band of 500 bp is amplified when the NH₂-terminal insert of 210 nucleotides is present (Xp120iso1). The band of 290 bp represents the shorter isoform of Xp120 (Xp120iso2). An RT-PCR reaction of elongation factor 1 (EF1a) was used as a loading control.

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