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Fig. 4. Knockdown of p120 expression results in the downregulation of C- and E-cadherin proteins but not of N-cadherin. (A) An amount of 20 ng or 40 ng of control MO or p120 MO was injected at the 1-cell stage. Embryos were lysed at stages 10.5, 15 and 22 and analyzed for expression of p120 and C-, E- and N-cadherin. Injection of p120 MO leads to a dose-dependent downregulation of p120 (arrow). The strong band indicated by the open arrowhead is nonspecific and serves as a loading control. Next to a loss of p120 expression, lower levels of C-cadherin and E-cadherin can be observed. Levels of N-cadherin are not affected. (B) RNA (500 pg) encoding mE-cadherin or Myc-tagged XN-cadherin was injected at the 1-cell stage together with control MO or p120 MO (20 ng). Embryos were lysed at stages 15 and 17 and analyzed for expression of ectopic mE-cadherin and Myc-tagged XN-cadherin. While the expression levels of mE-cadherin are dramatically reduced upon the co-injection of p120 MO, the levels of ectopic XN-cadherin are not affected.
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