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Fig. 5. Expression of p120-uncoupled E-cadherin affects the size of the eyes and cartilage. (A) Embryos were injected at the 8-cell stage in the dorsal-animal region and scored at stage 42 for the eye phenotype (a,c) and stage 46 for the cartilage phenotype after Alcian Blue staining (b,d). Tadpoles injected with 2 ng XE-cadh753AAA showing one smaller eye (c,d) compared with control injected embryos (a,b). (B) Graph representing the effect of XE-cadh753AAA RNA on the size of the craniofacial cartilage. Three normalized experiments are represented in one graph. The size of the head was calculated as in the legend of Fig. 3. The effect of XE-cadh753AA can be partially rescued by co-injection of 150 pg wild type (WT) RhoA or 50 pg dominant negative (DN) Rac (values are statistically different from those injected with XE-cadh753AAA RNA alone).





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