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Fig. 2. The C2 domains of the class I Rab11-FIPs bind PtdIns(3,4,5)P₃ and PA. (A) PipArrays^TM were overlayed with the indicated GST fusion proteins and immunoblotted with anti-GST antibody. (B) Densitometry of the PipArrays indicating that the class I Rab11-FIPs bind preferentially to PtdIns(3,4,5)P₃. The results are the mean of three independent experiments. (C) Nitrocellulose was spotted with serial dilutions of PS, PA, PC and PE. They were subsequently overlayed with the indicated GST fusion proteins and immunoblotted with anti-GST antibody. (D) Densitometry of the protein-lipid assay, indicating a preference for PA. The results are representative of three independent experiments.

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