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Fig. 7. (A) Rab11 translocates to the plasma membrane with class-I Rab11-FIPs. A431 cells expressing GFP-Rip11 were treated with either solvent or 1 µM PMA for 30 minutes at 37°C. The cells were then fixed and labelled with an antibody to Rab11. In unstimulated cells, GFP-Rip11 and Rab11 colocalize on intracellular vesicles and to the ERC. In PMA-treated cells, they display extensive colocalization at the plasma membrane. (B) A431 cells were treated with DMSO solvent alone or with 1 µM PMA for 30 minutes at 37°C. The cells were then fixed and labelled for RCP and the TfnR. In contrast to RCP-positive vesicles, TfnR-positive vesicles do not translocate to the plasma membrane. Scale bar, 10 µm.





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