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Fig. 8. The class I Rab11-FIPs localize with Akt to the plasma membrane in EGF-treated cells. A431 cells co-expressing HA-Akt and the indicated GFP-fused class I Rab11-FIP were treated with 100 ng ml1 EGF for 20 minutes at 37°C. The cells were fixed and labelled with an anti-HA antibody. The insets display regions of colocalization at the plasma membrane. Scale bar, 10 µm.