JCS01291 Supplemental Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Clustering of the 1412 most changing genes. Clustering has been performed using an unsupervised approach and applying the clustering algorithms provided by GeneSpring. A combination of two hierarchical clustering analyses (gene tree and condition tree) is shown. The gene tree is shown on the left; the condition tree on top. On the right, a magnification of the yellow box drawn on the left, shows genes clustered as expressed in all A clones. Gene colouring was based on normalized signals as shown at the bottom of the Figure.

• Supplemental Figure 2 -

  Fig. S2. Gene Ontology (GO) mapping. The analysis was performed setting the ‘probe set-count threshold’ to 23. This parameter permits the inclusion in the graph of only the GO nodes that have the chosen level of significance based on the number of probe sets read from the gene list data set. The node colours in the graphs are based on the percentage of probe sets on the MG-U74A array that mapped in this category; i.e. the percentage of mapped probe sets in individual categories that passed the statistical threshold. The results in the graph are coloured on a gradient scale from red to blue: red indicates the category with the maximum percentage of probe sets, whereas blue indicates the category with the minimum percentage of probe sets. Categories have the same colour if the percentage of probe sets is identical. The colour scale is calibrated to fit each GO branch in each probe list, and the intermediate results are coloured accordingly.

• Supplemental Figure 3 -

  Fig. S3. Developmental genes in mesoangioblasts. Embryonic tissue distribution of genes preferentially expressed in mesoangioblasts. Genes preferentially expressed in mesoangioblasts, for which in situ hybridization data were available in the literature, were analysed for their tissue distribution during embryogenesis. Ubi (ubiquitous) refers to genes expressed in at least one tissue of all three germ layers; neuro (genes expressed only in neural tissues); meso (genes expressed only in the mesoderm); endo (genes expressed only in endoderm); neuro-meso (genes expressed only in neural and mesodermal tissues); meso-endo (genes expressed only in mesoderm and endoderm); neuro-ecto (genes expressed only in neural tissues and ectoderm); ecto (genes expressed only in ectoderm).