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Fig. 3. Abortive neural differentiation of mesoangioblasts. (a) Immunofluorescence analysis of a co-culture of GFP expressing mesoangioblasts with differentiating cells from E10 embryonic neural tube, under neural stem cell differentiation conditions. Cultures were stained with TuJ1, an antibody against ß III-tubulin (red). GFP-positive cells appear green mainly in the nucleus where strong staining for ß III-tubulin is not present. (b) Immunofluorescence analysis of mesoangioblasts, cultured under neural stem cell differentiation conditions and stained with anti-GFAP (red); nuclei were stained with DAPI (blue). (c,d) Whole-mount fluorescence analysis of an E5 chick embryo brain injected with GFP-labelled mesoangioblasts. Clusters of GFP-positive cells can be detected both in forebrain (fb) and hindbrain where they appear to be restricted to specific rhombomeres (rh). Scale bar in c: 50 µm for a; 75 µm for b; 600 µm for c and d.





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