spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. TGFß, BMP and insulin mediated differentiation of mesoangioblasts. (a,b) Schematic representation of the TGFß/BMP (a) and insulin (b) signalling pathways in mesoangioblasts: the colour codes indicate the abundance of the different pathway components, ranging from red (highly expressed) to pale red to orange to yellow (poorly expressed); white: not in the array. (c) Western blot analysis of cell lysates from mesoangioblasts treated or untreated (C) with TGFß1, BMP2, or insulin; antibodies against TGFßRI, TGFßRII, BMPR1A, Smad1, Smad2/3, or IGF1Rß were used. (d) Histogram representing the percentage of mesoangioblasts staining Red Oil positive, after treatment with insulin-containing medium. (e-g) Immunofluorescence analysis of mesoangioblasts treated with TGFß1 and stained with antibodies against smooth muscle {alpha}-actin ({alpha}SMA; e), SM22 (f), calponin 1 (g) and smooth muscle myosin (SM-MyoHC; h) (all in green), merged with DAPI-stained nuclei (blue). (i) AP and Von Kossa staining of mesoangioblasts treated with BMP2 under osteogenic-promoting condition. Arrows point to calcified nodules. (j) Red Oil staining of mesoangioblasts treated with insulin under adipogenic-promoting condition. Scale bar in j: 50 µm for e-h; 200 µm for i; 150 µm for j.





Right arrow Return to article