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Fig. 5. Inhibition of the TGFß and BMP2 differentiation pathway. (a-c) Immunofluorescence analysis of control mesoangioblasts (a), mesoangioblasts treated with TGFß1 (b), or treated with the neutralizing anti-TGFbRII antibody prior to the addition of TGFß1 (c). The antibody to the smooth muscle marker Calponin 1 was used throughout. (d) Histogram representing the percentage of mesoangioblasts expressing Calponin 1 in the indicated culture conditions. (e-g) AP staining of control mesoangioblasts (e), mesoangioblasts treated with BMP2 (f), or transfected with noggin expression vector prior to the addition of BMP2 (g). (h) Histogram representing the percentage of AP-positive mesoangioblasts in the indicated culture conditions. Scale bar in e: 150 µm for a-c; 200 µm for d-f.





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