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Fig. 6. Wnt pathway and proliferation in mesoangioblasts. (a) Schematic representation of the Wnt signalling pathway in mesoangioblasts; colour codes as in Fig. 4. (b) Western blot analysis of cell lysates from the mesoangioblast clones A2 and A4, using antibodies against Wnt5a, Frizzled and ß-catenin. (c) Western blot analysis of concentrated culture medium from Frzb1-HA transfected 293T cells, using anti-HA antibody. (d) Growth curve of mesoangioblasts exposed to different concentrations of Frzb1 (pink and yellow lines, respectively, representing 50% and 100% of added medium from Frzb1-HA transfected 293T cells) and in control culture conditions (blue line).





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