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Fig. 5. Kinesin I heavy chain and dynactin complex interact in HeLa cell extract. (A) p150^Glued was precipitated from HeLa cell extracts by a monoclonal antibody and blots were probed with a monoclonal anti-kinesin-heavy-chain antibody (SUK4) right panel) or a monoclonal anti-p50 antibody (left panel). Control samples (-Cont.) were precipitated by an unrelated monoclonal antibody. Five µg of protein were loaded from the cell homogenate; the equivalent to 35 µg protein was used as starting material for immunoprecipitation and an equal amount of protein was loaded from the immunoprecipitated samples (lanes p150^Glued and -Cont.). (B) Depolymerisation of actin filaments partially restores the normal distribution of mitochondria in p50-expressing cells. Cells were co-transfected with 0.5 µg mito.DsRed and 1 µg p50.EGFP. Twenty hours after transfection cells were incubated with latrunculin B (25 µg ml^–1) in KRH buffer at 37°C and then imaged on an Ultra VIEW^TM Live Cell Confocal Imaging system. Mitochondria of the same cell (a) before the addition of latrunculin B, (b) after a 30-minute incubation or (c) after a 60 minute-incubation with the drug.

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