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Fig. 6. Mitochondrial movements in live HeLa cells. Cells were co-transfected with 0.25 µg mito.DsRed and (A) 1 µg padTrack-CMV or (B,C) p50.EGFP. Twelve hours after transfection, cells were imaged on an UltraVIEW^TM Live Cell Confocal Imaging system in KRH buffer at 37°C; images were acquired at 2 Hz. Boxed regions on the control cell (A) are enlarged in panels a and b. Indicated by the white arrowheads is a single moving mitochondrion whose original starting position is labeled with a red asterisk. Direction of movement is indicated by yellow arrows. (B,C) Mitochondrial distribution in HeLa cells expressing moderate (B) and high (C) levels of p50.EGFP. Note the long interconnected mitochondria in bottom panels Ba and b, and the collapsed mitochondrial structure in bottom panel Cc. (D) Twenty-five mitochondrial regions were selected in ten control cells (black bars), and in cells with moderate (grey bars) and high (white bars) p50 expression and movements of mitochondria were analysed (see Materials and Methods, and Results). Movements of mitochondria are most apparent on dynamic images (see supplementary material Movies 1-5).

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