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Fig. 7. Subcellular localisation of endogenous Drp1 in HeLa cells. HeLa cells were first fixed with cold methanol-acetone (1:1) and endogenous Drp1 and tubulin were detected by immunofluorescence with an anti-Drp1 (1:250) and an anti-{alpha}-tubulin (1:1000) antidody. (a) mito.DsRed; (b,f) Drp1; and (e) {alpha}-tubulin-immunofluorescence; (c,g) overlay of a and b, and e and f, respectively. Boxed areas in c and g are shown on an expanded scale in I, and II and III respectively. the enlarged image (III) was rotated 90° clockwise. Arrows indicate Drp1 assoc iation with punctate vesicular structures that are aligned along mitochondria (I) and microtubules (II,III).





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