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Fig. 7. CAR co-IP with MAGI-1b, PICK1, and PSD-95. Panel A shows western blots of COS-7 cells co-transfected with CAR and GFP-tagged MAGI-1b, PICK1, or PSD-95. IP was performed using a GFP Ab, to confirm the presence of the PDZ-domain-containing protein, the CAR specific monoclonal Ab RmcB and an unrelated control Ab (IgG or myc). Bands with the appropriate molecular weight were present for each of the PDZ-domain-containing proteins after IP with both GFP and RmcB but not the control Ab, indicating co-IP with CAR. Panel B shows western blots of COS-7 cells co-transfected with MAGI-1b-GFP and CAR or CAR{Delta}GSIV. IP for the FLAG epitope on CAR or CAR{Delta}GSIV resulted in a co-IP between MAGI-1b-GFP and CAR but not CAR{Delta}GSIV.





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